The preservation method of goose ELISA Kit
Guide: the problem of how to preserve goose ELISA kit has been puzzling many people, because goose ELISA kit is a perishable reagent. If it is not in a specific environment, it cannot be guaranteed that goose ELISA kit will be preserved for a longer time. In order to preserve goose ELISA kit for a longer time, researchers are constantly looking for methods
scientists are also interested in how cells keep young. They are studying how telomeres help repair damaged cells and how they play a key role in cancer research
scientists believe that telomere has a limitation that it is only beneficial to divisible cells, while most neurons are non divisible. As an important means to achieve precision extrusion, the use of melt gear pump must be strengthened for development and research. Neurons are the main constituent cells of the brain. If scientists can find a way to let telomeres enter even a small part of divisible brain cells, it can bring benefits to brain cells
Biologists say that telomeres are not the only cause of aging. Telomeres play up to 10% of the role in human aging. However, if cells can be healthier for a long time, diseases that cause serious lesions may be delayed, such as from 65 to 75 years oldif telomere therapy is used for short-term treatment of specific cells, for example, a week or two, it may have a successful therapeutic effect, which can repair chromosome telomeres and maintain cell division function. This treatment method can be used to cultivate patients' own cells in the laboratory, and can help the diseases with damaged cells and exhausted telomere quota, such as anemia, skin ulcer and inflammation. Many cancer researchers are trying to study how to treat cancer by blocking telomeres
and stem cells also have their own set in maintaining youth. They use the stem cell microenvironment. The research of Harvard University with water content ≤ 0.10% found the correlation between aging and changes in the stem cell microenvironment. The cell-shaped cradle like environment around stem cells protects stem cells, which is called niche. Niche not only provides nutrients for stem cells, but also guides the actions of stem cells and determines the differentiation direction of stem cells
secretions and excreta can be used as samples to determine some antibody or antigen components. Some samples can be determined directly (such as serum and urine), while others need to be pretreated (such as feces and some secretions). Most ELISA tests are based on serum samples. In addition to fibrinogen and anticoagulant, other components in plasma are equal to serum. The preparation of plasma samples requires the help of anti coagulants, especially thermoplastic composite coagulants, and the serum standard can be obtained as long as the elongation is converted into the tensile value after the natural coagulation of the serum and the contraction of the blood clot
except for special cases, serum is used as the test sample in medical examination. Plasma and serum can be used equally in ELISA. Serum samples can be collected by conventional methods, and hemolysis should be avoided. Substances with peroxidase activity will be released when erythrocytes are dissolved. In the ELISA determination marked with HRP, hemolytic samples may increase nonspecific color development. Serum samples should be tested when fresh. In case of bacterial contamination, the bacteria may contain endogenous HRP, which will also produce false positive reaction. If it is stored in the refrigerator for too long, it can polymerize, and the background can be deepened in indirect ELISA
generally speaking, serum samples measured within 5 days can be placed at 4 ℃, and those measured more than a week need to be frozen at low temperature. After the thawing of frozen serum, the protein is locally concentrated and unevenly distributed. It should be fully mixed, preferably gently, to avoid bubbles. It can be mixed upside down, and do not oscillate strongly on the mixer. Turbid or precipitated serum samples should be centrifuged or filtered first, and then tested after clarification. Repeated freezing and thawing will cause the antibody titer to drop, so if the serum samples for antibody testing need to be stored for multiple tests, it is advisable to pack a small amount of ice. Attention should be paid to the preservation of serum self collection
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